Selected Publications

Smirnova I, Kasho V, Jiang X, Pardon E, Steyaert J, Kaback HR., 2015. Transient conformers of LacY are trapped by nanobodies. Proc. Natl. Acad. Sci. USA 112(45):13839-44.
Link to Paper

Kumar, H., Finer-Moore, J., Kaback, HR., and Stroud, RM., 2015. Structure of LacY with an α-substituted galactoside: Connecting the binding site to the protonation site. Proc. Natl. Acad. Sci. USA 112(29): 9004–09.
Link to Paper

Kaback, HR., 2014. A chemiosmotic mechanism of symport. Proc. Natl. Acad. Sci. USA 112(5), 1259–64.
Link to Paper

Smirnova, I., Kasho, V., and Kaback, HR., 2014. Real-time conformational changes in LacY. Proc. Natl. Acad. Sci. USA 111(23):8440-5. Link to Paper

Serdiuk, T., Madej, MG., Sugihara, J., Kawamura, S., Mari, SA.,  Kaback, HR., and Müller, DJ. 2014, Substrate-induced changes in the structural properties of LacY.   Proc. Natl. Acad. Sci. USA 111(16):E1571-80.
Link to Paper

Kumar, H., Kasho, V., Smirnova, I., Finer-Moore, J., Kaback, HR., and Stroud, RM. 2014. Structure of Sugar-Bound LacY. Proc. Natl. Acad. Sci. USA111(5):1784-8. Link to Paper

Madej, MG., Kaback, HR. 2014. The Life and Times of Lac Permease: Crystals Ain’t Everything, but They Certainly Do Help. In: Membrane Transporter Mechanism: Ziegler, C. & Kraemer, R., eds. Springer Series in BiophysicsVolume 17, pp 121-158.

Smirnova, I., Kasho, V., Sugihara, J., Kaback, HR. 2013. Trp replacements for tightly interacting Gly-Gly pairs in LacY stabilize an outward-facing conformation. Proc. Natl. Acad. Sci. USA 110(22):8876-81. Link to Paper

Madej, MG., Dang, S., Yan, N., Kaback, HR. 2013. Evolutionary mix-and-match with MFS transporters. Proc. Natl. Acad. Sci. USA110(15);5870-5874. Link to Paper

Smirnova, I., Kasho, V., Sugihara, J., Kaback, H.R.  Opening the periplasmic cavity in lactose permease is the limiting step for sugar binding. Proc. Natl. Acad. Sci. USA, 2011 Sept.; 108(37):15147-15151. Link to Paper

Smirnova, I., Kasho, V., Choe, J.-Y., Altenbach, C., Hubbell, W. L. & Kaback, H.R. 2007. Sugar binding induces an outward facing conformation of LacY. Proc. Natl. Acad. Sci. USA104, 16504-9. Link to Paper

Guan, L., Mirza, O., Verner, G., Iwata, S. & Kaback, H.R. 2007. Structural determination of wild-type lactose permease. Proc. Natl. Acad. Sci. USA 104, 15294-8. Link to Paper

Kaback, H.R., Dunten, R., Frillingos, S., Venkatesan, P., Kwaw, I., Zhang, W. & Ermolova, N. 2007. Site-directed alkylation and the alternating access model for LacY. Proc. Natl. Acad. Sci. USA104, 491-4. Link to Paper

Abramson, J., Smirnova, I., Kasho, V., Verner, G., Kaback, H.R. & Iwata, S. 2003. Structure and Mechanism of the Lactose Permease of Escerichia coli. Science. 301: 610-615.
Link to Paper

Frilingos, S., Sahin-Toth, M., Wu, J., & Kaback, H.R., 1998.  Cys-Scanning Mutagenesis: a Novel Approach to Structure-Function Relationships in Polytopic Membrane Proteins. FASEB Journal , 12: 1281-1299. Link to Paper

Foster, D. L., Boublik, M. & Kaback, H. R.  1983.  Structure of the lac carrier protein of Escherichia coli.  J. Biol. Chem. 258: 31-34. Link to Paper

Newman, M. J., Foster, D., Wilson, T. H. & Kaback, H. R.  1981.  Purification and reconstitution of functional lactose carrier from Escherichia coli.  J. Biol. Chem. 256: 11804-11808. Link to Paper

Ramos, S., Schuldiner, S. & Kaback, H. R.  1976.  The electrochemical gradient of protons and its relationship to active transport in Escherichia coli  membrane vesicles.  Proc. Natl. Acad. Sci. USA 73: 1892-1896. Link to Paper

Kaback, H. R.  1971.  Bacterial membranes. Methods Enzymol. 22: 99-120.

Kaback, H. R.  1968.  The role of the phosphoenolpyruvate-phosphotransferase system in the transport of sugars by isolated membrane preparations of Escherichia coll.  J. Biol. Chem. 243: 3711-3724. Link to Paper